ZymoBIOMICS 96 MagBead Kit (No Lysis Matrix)
产品型号:Cat #: 11-696
简介:


Zymo Research
2 x 96 Preps/Unit
Brand: Zymo Research
High throughput purification of high quality, inhibitor free DNA from any sample including feces, soil, water, biofilms, swabs, sa

ZymoBIOMICS 96 MagBead Kit (No Lysis Matrix)  的详细介绍

The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20kb.

Brand Zymo Research Corporation
Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume >/= 37.5µl DNA Elution Buffer or DNase free water
Equipment Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block
Processing Volume Fecal </=100 mg, Soil </= 200 mg, Cells </= 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells)
Purity High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
Sample Storage Eluted DNA should be stored at </= -20°C.
Size Range Typically 15-20kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Type Total DNA
Yield Up to 10µg total DNA can be eluted into 50µl (37.5µl minimum)


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